A phytochemical and computational study on flavonoids isolated from Trifolium resupinatum L. and their novel hepatoprotective activity.
Identifieur interne : 000C05 ( Main/Exploration ); précédent : 000C04; suivant : 000C06A phytochemical and computational study on flavonoids isolated from Trifolium resupinatum L. and their novel hepatoprotective activity.
Auteurs : Emadeldin M. Kamel [Égypte] ; Ayman M. Mahmoud [Égypte] ; Sayed A. Ahmed [Égypte] ; Al Mokhtar Lamsabhi [Espagne]Source :
- Food & function [ 2042-650X ] ; 2016.
Descripteurs français
- KwdFr :
- Agents protecteurs (), Agents protecteurs (administration et posologie), Agents protecteurs (isolement et purification), Animaux, Antioxydants (), Antioxydants (administration et posologie), Antioxydants (isolement et purification), Composés phytochimiques (), Composés phytochimiques (isolement et purification), Composés phytochimiques (pharmacologie), Extraits de plantes (), Extraits de plantes (administration et posologie), Extraits de plantes (isolement et purification), Flavonoïdes (), Flavonoïdes (administration et posologie), Flavonoïdes (isolement et purification), Foie (), Foie (métabolisme), Humains, Mâle, Rat Wistar, Rats, Stress oxydatif (), Structure moléculaire, Trifolium ().
- MESH :
- administration et posologie : Agents protecteurs, Antioxydants, Extraits de plantes, Flavonoïdes.
- isolement et purification : Agents protecteurs, Antioxydants, Composés phytochimiques, Extraits de plantes, Flavonoïdes.
- métabolisme : Foie.
- pharmacologie : Composés phytochimiques.
- Agents protecteurs, Animaux, Antioxydants, Composés phytochimiques, Extraits de plantes, Flavonoïdes, Foie, Humains, Mâle, Rat Wistar, Rats, Stress oxydatif, Structure moléculaire, Trifolium.
English descriptors
- KwdEn :
- Animals, Antioxidants (administration & dosage), Antioxidants (chemistry), Antioxidants (isolation & purification), Flavonoids (administration & dosage), Flavonoids (chemistry), Flavonoids (isolation & purification), Humans, Liver (drug effects), Liver (metabolism), Male, Molecular Structure, Oxidative Stress (drug effects), Phytochemicals (chemistry), Phytochemicals (isolation & purification), Phytochemicals (pharmacology), Plant Extracts (administration & dosage), Plant Extracts (chemistry), Plant Extracts (isolation & purification), Protective Agents (administration & dosage), Protective Agents (chemistry), Protective Agents (isolation & purification), Rats, Rats, Wistar, Trifolium (chemistry).
- MESH :
- chemical , administration & dosage : Antioxidants, Flavonoids, Plant Extracts, Protective Agents.
- chemical , chemistry : Antioxidants, Flavonoids, Phytochemicals, Plant Extracts, Protective Agents.
- chemical , isolation & purification : Antioxidants, Flavonoids, Phytochemicals, Plant Extracts, Protective Agents.
- chemistry : Trifolium.
- drug effects : Liver, Oxidative Stress.
- metabolism : Liver.
- chemical , pharmacology : Phytochemicals.
- Animals, Humans, Male, Molecular Structure, Rats, Rats, Wistar.
Abstract
Plants from the genus Trifolium have been utilized in the treatment of chronic diseases by many cultures. The aim of this study was to investigate the antioxidant activity of flavonoids isolated from Trifolium resupinatum L. using an in vitro radical scavenging assay and a computational study of the structure-antioxidant activity relationship (SAR). A phytochemical fractionation of the ethanolic extract of T. resupinatum resulted in the isolation of one new flavonoid along with six known flavonoids. The structure of the isolated compounds was elucidated by data obtained from UV, IR, MS, 1D NMR and 2D NMR spectra. The order of antioxidant efficacies of the isolated flavonoids, obtained by DPPH assay, was correlated to that predicted by the computational analysis. To verify the antioxidant potential, the effect of T. resupinatum on oxidative stress, and the expression of the antioxidant enzymes and the redox sensitive nuclear factor erythroid 2-related factor 2 (Nrf2), in a cyclophosphamide-induced hepatotoxicity rat model was investigated. T. resupinatum protected against cyclophosphamide-induced liver injury through attenuation of oxidative stress and inflammation, and up-regulation of Nrf2. Therefore, the present study provides the first clarification of the detailed antioxidant SARs of T. resupinatum flavonoids and points to the involvement of Nrf2 in their hepatoprotective activity.
DOI: 10.1039/c6fo00194g
PubMed: 27053086
Affiliations:
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The aim of this study was to investigate the antioxidant activity of flavonoids isolated from Trifolium resupinatum L. using an in vitro radical scavenging assay and a computational study of the structure-antioxidant activity relationship (SAR). A phytochemical fractionation of the ethanolic extract of T. resupinatum resulted in the isolation of one new flavonoid along with six known flavonoids. The structure of the isolated compounds was elucidated by data obtained from UV, IR, MS, 1D NMR and 2D NMR spectra. The order of antioxidant efficacies of the isolated flavonoids, obtained by DPPH assay, was correlated to that predicted by the computational analysis. To verify the antioxidant potential, the effect of T. resupinatum on oxidative stress, and the expression of the antioxidant enzymes and the redox sensitive nuclear factor erythroid 2-related factor 2 (Nrf2), in a cyclophosphamide-induced hepatotoxicity rat model was investigated. T. resupinatum protected against cyclophosphamide-induced liver injury through attenuation of oxidative stress and inflammation, and up-regulation of Nrf2. Therefore, the present study provides the first clarification of the detailed antioxidant SARs of T. resupinatum flavonoids and points to the involvement of Nrf2 in their hepatoprotective activity. </div></front></TEI><affiliations><list><country><li>Espagne</li><li>Égypte</li></country><region><li>Communauté de Madrid</li></region><settlement><li>Madrid</li></settlement><orgName><li>Université autonome de Madrid</li></orgName></list><tree><country name="Égypte"><region name="Communauté de Madrid"><name sortKey="Kamel, Emadeldin M" sort="Kamel, Emadeldin M" uniqKey="Kamel E" first="Emadeldin M" last="Kamel">Emadeldin M. Kamel</name></region><name sortKey="Ahmed, Sayed A" sort="Ahmed, Sayed A" uniqKey="Ahmed S" first="Sayed A" last="Ahmed">Sayed A. Ahmed</name><name sortKey="Mahmoud, Ayman M" sort="Mahmoud, Ayman M" uniqKey="Mahmoud A" first="Ayman M" last="Mahmoud">Ayman M. Mahmoud</name></country><country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Lamsabhi, Al Mokhtar" sort="Lamsabhi, Al Mokhtar" uniqKey="Lamsabhi A" first="Al Mokhtar" last="Lamsabhi">Al Mokhtar Lamsabhi</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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